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. 2016 Jun 1;8(1):49–57. doi: 10.1080/21541248.2016.1194952

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — A Cdc42-defective binding mutant of Cdc42EP3 acts as a dominant-negative when expressed in cancer-associated fibroblasts (CAFs). (A) Panels show GFP (green), F-actin (magenta) and DAPI (blue) staining of cancer-associated fibroblasts (CAFs) following transfection with GFP or GFP-tagged wild-type (wt) or IS mutant (IS) Cdc42EP3 (CEP3) proteins. The grayscale panels show individual channel magnifications of perinuclear areas. Scale bars, 25 μm. (B) Panels show GFP (green), SEPT2 (magenta) and DAPI (blue) staining of CAFs following transfection with GFP or GFP-tagged wild-type (wt) or IS mutant (IS) Cdc42EP3 (CEP3) proteins. The grayscale panels show individual channel magnifications of perinuclear areas. Scale bars, 25 μm. (C) Left panels show images of GFP (green), pS19-MLC2 (magenta) and DAPI (blue) staining of CAFs following transfection with GFP or GFP-tagged wild-type (wt) or IS mutant (IS) Cdc42EP3 (CEP3) proteins. Right panels show GFP (green), pY118-Paxillin (magenta) and DAPI (blue) staining of CAFs following transfection with GFP or GFP-tagged wild-type (wt) or IS mutant (IS) Cdc42EP3 (CEP3) proteins. Scale bars represent 25 μm.