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HPA staining (red) was detected in (a) control, (b) air-lifted, (c) partially desiccated, and (d) IL-13-treated constructs. Scale bar: 50 μm. (e) MUC5AC levels secreted by goblet cells present in the constructs were analyzed by ELISA. Each of the experimental treatments significantly increased secretion compared to control cultures. Mean values from 6 independent experiments. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.005.
