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. 2017 Mar 1;12(3):e0172575. doi: 10.1371/journal.pone.0172575

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© 2017 Cannito et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — A,B. Immunohistochemistry analysis for NLRP3 on liver specimens from WT mice fed with MCS diet or MCD diet for 2wks, 4wks and 8wks (A) as well as WT mice and Casp 3^-/- knockout mice fed for 6wks (B). C. Image analysis quantification for NLRP3 staining (Fig 2B) as evaluated with Imagej software in liver sections from WT or caspase 3 -/- mice fed a MCD diet for 6 weeks. D,E. Immunohistochemistry analysis for F4/80 on liver specimens from WT mice fed with MCS diet or MCD diet for 2wks, 4wks and 8wks (D) as well as WT mice and Casp 3^-/- knockout mice fed for 6wks (E). F. Immunohistochemistry analysis for NLRP3 and CD68 on liver specimens from human NAFLD/NASH-related advanced fibrosis (F3 and F4). Original magnification as indicated.