Table 2. The top 76 hits were re-screened in a Vero vaccine cell line utilizing SMARTpools.
| Gene Target | Entrez ID | Deconvoluted siRNA | Catalog number | Fold changes (versus NTC) |
|---|---|---|---|---|
| The top 20 hits that repeated the primary screen’s findings were subjected to deconvolution studies. The data for these hits is outlined here. Data is presented as fold change compared to non-targeting siRNA control. | ||||
| None | NTC | D-001810-0X | 1 | |
| NEU2 | 4,759 | siRNA 1 | J-012058-17 | 1.196 |
| NEU2 | 4,759 | sIRNA 2 | J-012058-18 | 1.465 |
| NEU2 | 4,759 | siRNA 3 | J-012058-19 | 1.353 |
| NEU2 | 4,759 | siRNA 4 | J-012058-20 | 1.606 |
| NAT9 | 26,151 | siRNA 1 | J-017635-13 | 0.823 |
| NAT9 | 26,151 | sIRNA 2 | J-017635-14 | 1.241 |
| NAT9 | 26,151 | siRNA 3 | J-017635-15 | 1.484 |
| NAT9 | 26,151 | siRNA 4 | J-017635-16 | 1.442 |
| SVOPL | 1,36,306 | siRNA 1 | J-007346-13 | 0.777 |
| SVOPL | 1,36,306 | sIRNA 2 | J-007346-14 | 1.48 |
| SVOPL | 1,36,306 | siRNA 3 | J-007346-15 | 1.632 |
| SVOPL | 1,36,306 | siRNA 4 | J-007346-16 | 1.044 |
| COQ9 | 57,017 | siRNA 1 | J-022565-18 | 0.948 |
| COQ9 | 57,017 | sIRNA 2 | J-022565-19 | 1.531 |
| COQ9 | 57,017 | siRNA 3 | J-022565-20 | 1.477 |
| COQ9 | 57,017 | siRNA 4 | J-022565-21 | 1.26 |
| NDUFA9 | 4,704 | siRNA 1 | J-016044-09 | 1.085 |
| NDUFA9 | 4,704 | sIRNA 2 | J-016044-10 | 1.648 |
| NDUFA9 | 4,704 | siRNA 3 | J-016044-11 | 1.641 |
| NDUFA9 | 4,704 | siRNA 4 | J-016044-12 | 1.233 |
| RAD51AP1 | 10,635 | siRNA 1 | J-017166-05 | 1.094 |
| RAD51AP1 | 10,635 | sIRNA 2 | J-017166-06 | 1.217 |
| RAD51AP1 | 10,635 | siRNA 3 | J-017166-07 | 1.568 |
| RAD51AP1 | 10,635 | siRNA 4 | J-017166-08 | 1.478 |
| COX20 | 1,16,228 | siRNA 1 | J-021429-09 | 1.021 |
| COX20 | 1,16,228 | sIRNA 2 | J-021429-10 | 1.478 |
| COX20 | 1,16,228 | siRNA 3 | J-021429-11 | 1.602 |
| COX20 | 1,16,228 | siRNA 4 | J-021429-12 | 0.937 |
| MAPK6 | 5,597 | siRNA 1 | J-003594-11 | 1.361 |
| MAPK6 | 5,597 | sIRNA 2 | J-003594-12 | 1.405 |
| MAPK6 | 5,597 | siRNA 3 | J-003594-13 | 1.378 |
| MAPK6 | 5,597 | siRNA 4 | J-003594-14 | 1.067 |
| WDR62 | 2,84,403 | siRNA 1 | J-031771-09 | 1.361 |
| WDR62 | 2,84,403 | sIRNA 2 | J-031771-10 | 1.485 |
| WDR62 | 2,84,403 | siRNA 3 | J-031771-11 | 1.402 |
| WDR62 | 2,84,403 | siRNA 4 | J-031771-12 | 1.622 |
| LRGUK | 1,36,332 | siRNA 1 | J-015997-09 | 1.101 |
| LRGUK | 1,36,332 | sIRNA 2 | J-015997-10 | 1.585 |
| LRGUK | 1,36,332 | siRNA 3 | J-015997-11 | 1.161 |
| LRGUK | 1,36,332 | siRNA 4 | J-015997-12 | 1.568 |
| CDK6 | 1,021 | siRNA 1 | J-003240-10 | 1.315 |
| CDK6 | 1,021 | siRNA 2 | J-003240-11 | 1.434 |
| CDK6 | 1,021 | siRNA 3 | J-003240-12 | 1.529 |
| CDK6 | 1,021 | siRNA 4 | J-003240-13 | 0.951 |
| KIAA1683 | 80,726 | siRNA 1 | J-031251-05 | 1.158 |
| KIAA1683 | 80,726 | siRNA 2 | J-031251-06 | 1.242 |
| KIAA1683 | 80,726 | siRNA 3 | J-031251-07 | 1.24 |
| KIAA1683 | 80,726 | siRNA 4 | J-031251-08 | 1.097 |
| CRISP3 | 10,321 | siRNA 1 | J-020092-09 | 0.969 |
| CRISP3 | 10,321 | siRNA 2 | J-020092-10 | 1.454 |
| CRISP3 | 10,321 | siRNA 3 | J-020092-11 | 1.436 |
| CRISP3 | 10,321 | siRNA 4 | J-020092-12 | 1.218 |
| GRPR | 2,925 | siRNA 1 | J-005624-05 | 1.039 |
| GRPR | 2,925 | siRNA 2 | J-005624-06 | 1.535 |
| GRPR | 2,925 | siRNA 3 | J-005624-07 | 1.925 |
| GRPR | 2,925 | siRNA 4 | J-005624-08 | 0.971 |
| DPH7 | 92,715 | siRNA 1 | J-016561-09 | 1.121 |
| DPH7 | 92,715 | siRNA 2 | J-016561-10 | 1.343 |
| DPH7 | 92,715 | siRNA 3 | J-016561-11 | 1.573 |
| DPH7 | 92,715 | siRNA 4 | J-016561-12 | 1.275 |
| GEMIN8 | 54,960 | siRNA 1 | J-020841-17 | 1.1 |
| GEMIN8 | 54,960 | siRNA 2 | J-020841-18 | 1.047 |
| GEMIN8 | 54,960 | siRNA 3 | J-020841-19 | 1.501 |
| GEMIN8 | 54,960 | siRNA 4 | J-020841-20 | 1.437 |
| KIAA1407 | 57,577 | siRNA 1 | J-014068-17 | 1.154 |
| KIAA1407 | 57,577 | siRNA 2 | J-014068-18 | 1.292 |
| KIAA1407 | 57,577 | siRNA 3 | J-014068-19 | 1.424 |
| KIAA1407 | 57,577 | siRNA 4 | J-014068-20 | 1.089 |
| RFXAP | 5,994 | siRNA 1 | J-011104-05 | 1.005 |
| RFXAP | 5,994 | siRNA 2 | J-011104-06 | 1.514 |
| RFXAP | 5,994 | siRNA 3 | J-011104-07 | 1.532 |
| RFXAP | 5,994 | siRNA 4 | J-011104-08 | 1.669 |
| SMARCA4 | 6,597 | siRNA 1 | J-010431-05 | 1.493 |
| SMARCA4 | 6,597 | siRNA 2 | J-010431-06 | 1.51 |
| SMARCA4 | 6,597 | siRNA 3 | J-010431-07 | 1.462 |
| SMARCA4 | 6,597 | siRNA 4 | J-010431-08 | 1.698 |
| CFAP58 | 1,59,686 | siRNA 1 | J-026312-17 | 1.452 |
| CFAP58 | 1,59,686 | siRNA 2 | J-026312-18 | 1.4 |
| CFAP58 | 1,59,686 | siRNA 3 | J-026312-19 | 1.366 |
| CFAP58 | 1,59,686 | siRNA 4 | J-026312-20 | 1.558 |