Figure 2. RAN is a target of miR-134.

(a) Sequence of the putative binding site between miR-134 and 3′UTR of RAN. (b) and (c) Hep-2c cells were reverse-transfected with miR-134-5p or miR mimic non-targeting scrambled negative control (miR-NTC) at a final concentration of 25 nM. After 48 h, total RNA was isolated from HEp-2c cells, and then miR-134 (b) and RAN (c) mRNA levels were determined by qRT-PCR. The data were normalized to the 18S rRNA gene and were expressed as fold change relative to mock-transfected cells. The significant differences are compared with miR-control. Each experiment was performed in quadruplicate and each experiment was repeated twice. The results were reproduced and the data in the figure were representative of 8 plates. Error bars represent s.e.m. *P<0.05, ***P<0.001.