Figure 4. miR-134 targets EV71 replication in RD cells.

(a) Schematic of the experimental design. (b) RD cells were reverse-transfected with miR-134-5p or miR mimic non-targeting scrambled negative control (miR-NTC) at final concentration of 25 nM. 48 h later the cells were infected with EV71 at MOI of 0.01 for 24 h and the antiviral effect of miR-134 was determined by plaque assay. Error bars represent s.e.m. of of quadruplicate. Each experiment was performed in quadruplicate and each experiment was repeated twice. The results were reproduced and the data in the figure were representative of 8 plates. The significant differences are compared with miR-control. ***P<0.001.