Fig 3. Screening the Psa phenotype of interest (POI) library for mutants with altered motility.

(A) Mutants were screened on KB agar medium (left) and swimming agar medium (right). Representative results for POI library plate 62 (P62) are shown. Swimming mutants are denoted by a blue (super-swimming phenotype; position P62-D5) and red (non-swimming phenotype; position P62-E3) arrow. Note the significantly enlarged colony ring of the super-swimming mutant; (B) Schematic of the IYO_024315 and IYO_010080 genes, which are disrupted in motility mutants retrieved from positions P62-D5 and P62-E3, respectively in (A). Arrow colors correspond to the mutants highlighted in (A), and show the location of gene insertion by the transposon. All replica plating was carried out in triplicate.