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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Mol Biol Rep. 2011 Dec 13;39(5):5393–5399. doi: 10.1007/s11033-011-1339-4

Fig. 2.

Fig. 2

Promoter activity decreased by addition of (extending) a 121-bp fragment into Intron 1. a Schematic of 7.9 kb-Luc expression constructs and addition of a 121-bp fragment. b–d Addition of 121-bp fragment decreased the promoter activity in SK-N-AS, SH-SY5Y and HEK293T. Luc activity (arbitrary units) represents promoter activity of 7.9 kb hDAT fragment before (7.9 kb for pGL3-hDAT7.9kb) and after (7.9kb+121bp for pGL3-hDAT7.9kb+121bp) adding 121-bp. P < 0.0001 for SK-N-AS (N = 6) and SH-SY5Y (N = 14) or P = 0.0342 for HEK293T (N = 2 each in duplicate) all by t tests