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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Mol Biol Rep. 2011 Dec 13;39(5):5393–5399. doi: 10.1007/s11033-011-1339-4

Fig. 5.

Fig. 5

EMSA of nuclear protein binding to four subfragments (I–IV) of 121-bp. Nuclear proteins were isolated from SK-N-AS, BE(2)-M17, SH-SY5Y and HEK293T, as indicated on top. DNA sequences of I–IV are shown in Fig. 4a and ds oligos were labeled by 35S-dATP. Competitor is 30-fold excess of unlabeled double-strand oligo. Arrow specific binding activity by comparing to next lane with competitor; lowest band poly(A/T, generated by 35S-A filling-in)-binding activity (control not shown)