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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Mol Cell Cardiol. 2015 Nov 28;90:21–29. doi: 10.1016/j.yjmcc.2015.11.030

Fig. 8. PAR4 induces myocyte apoptosis through JNK pathway.

Fig. 8

NRCMs were pretreated with vehicle or 5 μM SP600125 for 30 minutes prior to treatment with 10 U/ml thrombin, PAR1/PAR2- or PAR4-AP for 24 h. (A) Cells were assessed for immunoblot analysis. Top: representative immunoblot with each lane from a single gel exposed for the same duration. Bottom: Quantification of experiments expressed as mean ± SEM from three separate cultures. (B) Representative images of total nuclei (blue) and apoptotic nuclei (Green) from untreated and AYPGKF treated myocytes in presence or absence of SP600125. Scale bar: 100 μm. (C) Quantification of percent TUNEL-positive nuclei. Samples were assayed in triplicate and results are representative of 3 independent experiments. (D) Lysates from NRCMs were assayed for caspase-3 activity assay. Data are mean ± SEM; *P<0.05 vs. control; †P<0.05 vs. treated myocytes.