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. 2016 Nov 4;125(3):392–399. doi: 10.1289/EHP54

Figure 1.

Figure 1

Study plan. The dotted lines indicate periods of exposure to cigarette smoke. At birth, 31 pups were analyzed in the Smoke group (weight n = 31, gene expression n = 24) and 29 in the Air group (weight n = 29, gene expression n = 21). On day 21, pooling two experiments (see Figures S1 and S2), 25 pups were analyzed in the Smoke group (weight n = 25, lung morphometry n = 17, lung mechanics n = 14, gene expression n = 8) and 26 in the Air group (weight n = 26, lung morphometry n = 10, lung mechanics n = 15, gene expression n = 8). On day 49, 11 pups were analyzed in the SS group (weight n = 11, lung morphometry n = 8, lung mechanics n = 8, gene expression n = 11), 11 in the SA group (weight n = 11, lung morphometry n = 8, lung mechanics n = 8, gene expression n = 11), 19 in the AS group (weight n = 19, lung morphometry n = 17, lung mechanics n = 8, gene expression n = 16), and 12 in the AA group (weight n = 12, lung morphometry n = 10, lung mechanics n = 8, gene expression n = 7). AA, Air–Air; AS, Air–Smoke; GD0, gestational day 0 (conception); PC14, pre-conception day 14 (14 days before conception); Pups D0, pups aged 0 day (birth); Pups D21, pups aged 21 days; Pups D49, pups aged 49 days; SA, Smoke–Air; SS, Smoke–Smoke.