Correlation of % Total Body Surface Area (TBSA) closed with % TBSA full-thickness burn, frequencies of regrafting prior to POD 28, and antibodies to the biopolymer scaffold. A) A strong positive correlation (R2 = 0.65, p<0.001) was detected between % TBSA closed with ESS at POD 28, and % TBSA full-thickness burn. B) Neither condition required total regrafting of comparative sites. ESS comparative sites required partial regrafting in 26.7% of the ESS procedures (4/15), and AG required no regrafting. C) Enzyme-Linked ImmunoSorbant Assay (ELISA) of pre-immune and post-immune sera. ELISA values (OD490) for subject sera to homogenized collagen-GAG matrices were not statistically different before or 28 days after one or more graftings of ESS. Control sera from rabbits immunized with homogenized matrices showed statistically significant increases of ELISA values.