Figure 11.

SDF-1-dependent migration of Lin⁻ CD123^low CD127^low cells may precede activation and local production of IL-17 and IL-22 in psoriasis patients. (A) Frequencies of Lin⁻ CD123^low and Lin⁻ CD127⁺ populations in control skin (CS) N = 7, non-lesioned (NL) skin N = 7, and lesioned (L) N = 15 skin from psoriasis patients. (B) Intracellular expression of IL-17 and IL-22 in Lin⁻ CD123^low cells from CS, NL and L skin cultured during 18 h ± IL-1β, IL-2, and IL-23 (ILC3 activation cocktail) ± PMA/Iono (last 6 h) Dot plots are representative of at least three independent experiments. FMO, fluorescence minus one control. (C,D) Percentages of skin IL-17⁺ and IL-22⁺ Lin⁻ CD123^low cells (CS: N = 7 and L: N = 12) or Lin⁻ CD127⁺ cells (CS: N = 4 and L: N = 11) cultured during 18 h ± IL-2, IL-23, and IL-1β (ILC3 activation cocktail) + PMA/Iono (*p < 0.05, **p < 0.01).