Figure 5.

Steady-state peripheral Lin⁻ CD123⁺ CD127⁺ population expresses limited T helper type cytokine variety. Percentages of positive and negative cells that express IFN-γ, IL-2, and IL-4; IL-17 and IL-22 in the presence of PMA/Iono (6 h) (A) Lin⁻ CD123⁺ and Lin⁻ CD127⁺ (B) populations within peripheral blood mononuclear cells. (C,D) IFN-γ expression in Lin⁻ CD123⁺ and Lin⁻ CD127 after 18 h of activation with the ILC1 cocktail (IL-12 + IL-15). In each panel, FMO controls are shown in the upper panels. Density plots are representative of at least three independent experiments. FMO, fluorescence minus one control.