Figure 6.

The Lin⁻ CD123⁺ CD127^low population in PB expresses high levels of IL-8. (A) IL-8 and IL-4 quantification in culture supernatants from isolated Lin⁻ CD123⁺ cells (within the lymphoid region gated as depicted in Figure S1A in Supplementary Material) cultured for 18 h ± IL-3, PMA/Iono or IL-3 + PMA/Iono. (B) Percentages of positive and negative cells that express IL-8 (lower panel) in the presence of PMA/Iono (6 h) in Lin⁻ CD123⁺ and Lin⁻ CD127⁺ populations within peripheral blood mononuclear cells (PBMCs). Upper panel: FMO control for each cell population. Dot plots are representative of at least three independent experiments. (C) Percentage of Lin⁻ CD123⁺ and Lin⁻ CD127⁺ IL-8⁺ cells within PBMCs ± IL-3 and PMA/Iono (n = 5). (D) Expression of IFN-γ, IL-2, IL-4, and IL-8 in sorted (as in Figure 1) in Lin⁻ CD123⁺ cultured in the presence of PMA/Iono for 6 h. Density plots are representative of at least three independent experiments. FMO, fluorescence minus one control (**p < 0.01).