Figure 8.

The Lin⁻ CD123⁺ CD127^low population from the lymphoid region after activation downregulates CD123 and basophil markers. (A) Prospective identification of two CD123⁺ cell populations in the FSC^int SSC^int and FSC^low SSC^low fractions as FSC^int SSC^int (1) and FSC^low SSC^low (2) after activation with PMA/Iono (6 h). (B,C) Basophil markers expression after PMA/Iono (6 h) stimulation in the Lin⁻ CD123⁺ (FSC^int SSC^int) and Lin⁻ CD123⁺ (FSC^low SSC^low). Red: non-stimulated and orange: stimulated Lin⁻ CD123⁺ (1: FSC^int SSC^int). Dark blue: non-stimulated and light blue: stimulated Lin⁻ CD123⁺ (2: FSC^low SSC^low). White: isotype. Median fluorescence intensity values representative of three independent experiments are presented. (D) 2D7 and IL-8 expression after PMA/Iono (6 h) stimulation or IgE crosslinking. Upper panel: Lin⁻ CD123⁺ (FSC^int SSC^int) and lower panel: Lin⁻ CD123⁺ (FSC^low SSC^low). FMO: for each population is shown at the right of each panel. Density plots are representative of at least three independent experiments. FMO, fluorescence minus one control.