Figure 4.

Gene expression analysis of engineered S. elongatus PCC 7942 using SyneBrick 2.0 vectors. (A) Growth of engineered S. elongatus PCC (pSe23Bb7kc-eYFP/P, pSe23Bb7.3kc-eYFP/P, and pSe23Bb7.4kc-eYFP/P) under 5% (v/v) CO₂ bubbling in BG-11 supplemented with indicated concentrations of IPTG, measured at OD₇₃₀ (black square, 0 mM; red circle, 0.01 mM; blue triangle, 0.1 mM; inverted pink triangle, 1 mM; green diamond, 10 mM). (B) Specific fluorescence (intensities per OD₇₃₀) were calculated for strains. Experiments were performed in triplicate cultures. All data are presented as mean ± standard deviation (SD) from triplicate cultures.