Skip to main content
. 2017 Mar 2;11:12. doi: 10.3389/fncir.2017.00012

Figure 4.

Figure 4

Challenges for simultaneous visualization of both presynaptic and postsynaptic activation. (A) Construct design. To visualize the synchrous firing of the presynaptic and the postsynaptic neurons, AS-PaRac1, neuronal filler, as well as presynaptic marker VAMP2-fused with mTurquoise (mTq-VAMP2) were transduced. For the selective labeling of the circuit of interest, CRE-dependent expression of the presynaptic marker (doublefloxed inverted open-reading-frame, DIO) was used. (B) Representative image of the simultaneous visualization of presynaptic and postsynaptic activation. The merged puncta (labeled by both, mTq and mRFP) are supposed to be Hebbian synaptic plasticity (arrowheads). Abbreviations: AS-PaRac1, Activated Synapse-targeting PhotoActivatable Rac1; mRFP, monomeric red fluorescent protein for visualization of neuronal morphology; VAMP2, vesicle-associated membrane protein 2.