Skip to main content
. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Mitochondrion. 2016 Aug 31;33:84–101. doi: 10.1016/j.mito.2016.08.017

Figure 1. Mitochondrial partners of cardiac mitoBKCa predict new channel functions.

Figure 1

(A) Gene ontology analysis using DAVID of mitochondrial BKCa proteome revealed mitoBKCa potential involvement with 13 mitochondrial functions in the heart. Numbers in parenthesis correspond to the number of proteins allocated to each group. “Transport” group was entered manually. Isolation of BKCa partners was performed using pull-down and immunoprecipitation with: 1) Recombinant GST-DEC and mitochondrial lysates of isolated cardiomyocytes, 2) Recombinant GST-DEC and whole-cardiomyocyte lysates, and 3) Monoclonal anti-BKCa antibody and lysates of Percoll-purified mitochondria from left ventricle. Controls included GST pull-down and IgG immunoprecipitation (see Methods). A total of 151 mitochondrial proteins with a score>21 were identified. (B) Example of mass spectrum of a peptide matching ANT.