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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Mitochondrion. 2016 Aug 31;33:84–101. doi: 10.1016/j.mito.2016.08.017

Figure 6. BKCa and ANT are imported into mitochondria of HEK293T cells.

Figure 6

A and B, Dually labeled immunoblots of BKCa (A) or BKCa-DEC (B) co-expressed with ANT show that the three constructs were imported into “crude” and purified mitochondria fractions. Note the enrichment of ANT in the purified mitochondria fraction. Labels are as in Fig. 3. BKCa was recognized with anti-HA mAb. ANT was recognized with anti-c-Myc pAb. C, D, and E, Quantification of BKCa, BKCa-DEC and ANT relative abundance in different cellular fractions. *, p<0.05 compared to CL; **, p<0.01compared to CL. n=3 independent experiments.