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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Mitochondrion. 2016 Aug 31;33:84–101. doi: 10.1016/j.mito.2016.08.017

Figure 7. Molecular determinant of BKCa channel interaction with ANT.

Figure 7

Different regions of BKCa and wild-type BKCa (see scheme in Fig. 4A) were either expressed alone or co-expressed with ANT. (A) Dually labeled immunoblot shows that immunoprecipitated ANT pulled down wild-type and deletion BKCa constructs (lanes 8-13) to different degrees. Squares and arrows mark the bands of the expected sizes. Dashed squares mark background signals, which were subtracted for the analysis in (C). ANT was immunoprecipitated with anti-DDK mAb. Coimmunoprecipitated BKCa proteins and ANT were detected with anti-c-Myc pAb. (B) Control immunoblot of input lysates of cells expressing different BKCa regions and ANT. (C) ANT efficiency in pulling different BKCa constructs indicates that BKCa channel interacts with ANT mainly through the transmembrane domain (construct 1-343). *, p<0.05 with respect to construct 1-343; #, p<0.05 with respect to construct 322-1113. n=3 independent experiments.