Table 2.
BKCa channel-interacting proteins from identified by LC/MS/MS.
| Proteins | Uniprot ID | Mass, Da | Score | Peptides |
|---|---|---|---|---|
| Endoplasmic Reticulum | ||||
| Extended synaptotagmin-1 (B,C) | ESYT1_RAT | 121369 | 139 | 6 |
| 78 kDa glucose-regulated protein (B,C) | GRP78_RAT | 72473 | 136 | 4 |
| Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (A, B,C) | AT2A1_RAT | 110707 | 65 | 2 |
| Carnitine O-acetyltransferase (A, C) | CACP_RAT | 71211 | 65 | 4 |
| Erlin-2 (B) | ERLN2_RAT | 37915 | 62 | 4 |
| Elongation factor 1-gamma (A,B) | EF1G_RAT | 50371 | 57 | 3 |
| Serpin H1 (B) | SERPH_RAT | 46602 | 51 | 3 |
| Cytochrome P450 2C11 (A) | CP2CB_RAT | 57658 | 39 | 3 |
| Calreticulin (A) | CALR_RAT | 48137 | 38 | 1 |
| Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 (B) |
RPN2_RAT | 69149 | 37 | 2 |
| Sterol regulatory element-binding protein 2 (A, C) | SRBP2_RAT | 124096 | 37 | 2 |
| Calnexin (A, B) | CALX_RAT | 67612 | 36 | 2 |
| ORM1-like protein 3 (C) | ORML3_RAT | 17451 | 34 | 1 |
| Reticulon-4 (C) | RTN4_RAT | 126766 | 33 | 3 |
| Antigen peptide transporter 2 (A) | TAP2_RAT | 78063 | 32 | 3 |
| Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 (A) | PLOD3_RAT | 85577 | 31 | 2 |
| Cytochrome P450 2A2 (A) | CP2A2_RAT | 56480 | 30 | 3 |
| 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4- isomerase type 2 (A) |
3BHS2_RAT | 42592 | 29 | 1 |
| HCLS1-associated protein X-1 (C) | HAX1_RAT | 31429 | 29 | 2 |
| Inositol 1,4,5-trisphosphate receptor type 1 (A, C) | ITPR1_RAT | 316486 | 29 | 3 |
| Transmembrane protein 214 (A) | TM214_RAT | 77486 | 29 | 2 |
| Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (A) |
NSDHL_RAT | 40671 | 27 | 1 |
| Squalene synthase (A) | FDFT_RAT | 48703 | 26 | 1 |
| Protein ERGIC-53-like (A) | LMA1L_RAT | 56571 | 26 | 2 |
| BET1 homolog (A) | BET1_RAT | 13336 | 26 | 2 |
| Protein ERGIC-53 (B) | LMAN1_RAT | 58206 | 26 | 1 |
| Cytochrome P450 3A1 (B) | CP3A1_RAT | 58222 | 26 | 1 |
| Carboxylesterase 3 (B) | CES3_RAT | 62393 | 25 | 2 |
| Choline-phosphate cytidylyltransferase B (B) | PCY1B_RAT | 42159 | 25 | 1 |
| Reticulocalbin-2 (A) | RCN2_RAT | 37410 | 25 | 2 |
| Cytochrome P450 2C12, female-specific (C) | CP2CC_RAT | 56453 | 24 | 1 |
| Cytochrome P450 2E1 (C) | CP2E1_RAT | 56990 | 24 | 2 |
| Multiple coagulation factor deficiency protein 2 homolog (A) | MCFD2_RAT | 16252 | 24 | 1 |
| Thrombospondin-4 (A) | TSP4_RAT | 110882 | 24 | 1 |
| DnaJ homolog subfamily C member 3 (A,C) | DNJC3_RAT | 57981 | 24 | 3 |
| Lipase maturation factor 2 (A) | LMF2_RAT | 80431 | 23 | 1 |
| Phosphatidylinositol-glycan biosynthesis class W protein (A,C) | PIGW_RAT | 57205 | 23 | 1 |
| LDLR chaperone MESD (A) | MESD_RAT | 25314 | 23 | 1 |
| Uncharacterized glycosyltransferase AER61 (C) | AER61_RAT | 62340 | 23 | 2 |
| Inhibitor of nuclear factor kappa-B kinase-interacting protein (B) |
IKIP_RAT | 42391 | 22 | 3 |
| Sphingosine-1-phosphate lyase 1 (A) | SGPL1_RAT | 64287 | 22 | 4 |
| Dehydrogenase/reductase SDR family member 7B (C) | DRS7B_RAT | 35662 | 22 | 2 |
| UDP-glucose:glycoprotein glucosyltransferase 1 (C) | UGGG1_RAT | 177061 | 22 | 3 |
| Poly [ADP-ribose] polymerase 16 (C) | PAR16_RAT | 37088 | 21 | 2 |
|
| ||||
| Golgi | ||||
| Ras-related protein Rab-10 (C) | RAB10_RAT | 23072 | 102 | 2 |
| Ras-related protein Rab-1A (C) | RAB1A_RAT | 22891 | 67 | 2 |
| Calcium-transporting ATPase type 2C member 1 (B) | AT2C1_RAT | 101519 | 39 | 2 |
| Nucleobindin-2 (A) | NUCB2_RAT | 50173 | 39 | 1 |
| Phosphofurin acidic cluster sorting protein 1 (A, B) | PACS1_RAT | 105034 | 32 | 2 |
| Golgin subfamily A member 2 (A) | GOGA2_RAT | 113232 | 31 | 7 |
| Caveolin-3 (A), B) | CAV3_RAT | 17904 | 31 | 1 |
| CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3- sialyltransferase (A) |
SIAT6_RAT | 42340 | 29 | 3 |
| Protein O-linked-mannose beta-1,2-N- acetylglucosaminyltransferase 1 (A) |
PMGT1_RAT | 75670 | 28 | 1 |
| Alpha-1,3-mannosyl-glycoprotein 2-beta-N- acetylglucosaminyltransferase (A) |
MGAT1_RAT | 51839 | 27 | 1 |
| Myomegalin (A, B,C) | MYOME_RAT | 263193 | 26 | 7 |
| Vacuolar protein sorting-associated protein 54 (B) | VPS54_RAT | 109913 | 24 | 3 |
| Acid phosphatase-like protein 2 (C) | ACPL2_RAT | 55664 | 24 | 1 |
| Golgi phosphoprotein 3-like (A) | GLP3L_RAT | 33072 | 23 | 3 |
| Carbohydrate sulfotransferase 11 (B) | CHSTB_RAT | 42112 | 23 | 3 |
| Beta-1,4-mannosyl-glycoprotein 4-beta-N- acetylglucosaminyltransferase (A) |
MGAT3_RAT | 62552 | 22 | 1 |
| Golgi SNAP receptor complex member 2 (B) | GOSR2_RAT | 24649 | 21 | 1 |
|
| ||||
| Nucleus | ||||
| Vimentin (A, B,C) | VIME_RAT | 53757 | 942 | 16 |
| Alpha-actinin-4 (A, B,C) | ACTN4_RAT | 105306 | 319 | 18 |
| Keratin, type I cytoskeletal 13 (A, C) | K1C13_RAT | 48098 | 208 | 9 |
| Lamin-B1 (A) | LMNB1_RAT | 66794 | 126 | 7 |
| Caveolin-2 (B) | CAV2_RAT | 18482 | 124 | 1 |
| Histone H2A type 1-C (A) | H2A1C_RAT | 14097 | 123 | 2 |
| Cullin-associated NEDD8-dissociated protein 2 (A) | CAND2_RAT | 141238 | 117 | 4 |
| 60S ribosomal protein L23a (A, C) | RL23A_RAT | 17684 | 104 | 1 |
| Homeobox protein Nkx-6.1 (A, B,C) | NKX61_RAT | 37723 | 89 | 1 |
| Cyclin-dependent kinase 7 (Fragment) (A) | CDK7_RAT | 37402 | 59 | 2 |
| DNA-directed RNA polymerase I subunit RPA1 (A, B,C) | RPA1_RAT | 196235 | 54 | 3 |
| Centrosomal protein of 57 kDa (A, C) | CEP57_RAT | 57404 | 49 | 4 |
| Heterogeneous nuclear ribonucleoproteins A2/B1 (A) | ROA2_RAT | 37512 | 49 | 1 |
| Serine/threonine-protein kinase TNNI3K (A, B) | TNI3K_RAT | 93985 | 44 | 1 |
| Mitotic spindle assembly checkpoint protein MAD2B (A, B) | MD2L2_RAT | 24615 | 39 | 1 |
| Speckle targeted PIP5K1A-regulated polyA polymerase (A) | STPAP_RAT | 95516 | 38 | 2 |
| Myc-induced nuclear antigen (A) | MINA_RAT | 53638 | 37 | 2 |
| DNA mismatch repair protein Msh2 (A) | MSH2_RAT | 104704 | 37 | 5 |
| Snurportin-1 (A) | SPN1_RAT | 41337 | 37 | 1 |
| Transcription initiation factor TFIID subunit 6 (A) | TAF6_RAT | 73295 | 36 | 1 |
| Ras association domain-containing protein 2 (B) | RASF2_RAT | 38168 | 36 | 1 |
| 60S ribosomal protein L11 (A) | RL11_RAT | 20468 | 36 | 1 |
| Serine/threonine-protein kinase MAK (A, C) | MAK_RAT | 70280 | 35 | 1 |
| Tyrosine-protein kinase Fer (Fragment) (A) | FER_RAT | 37479 | 35 | 2 |
| Coiled-coil and C2 domain-containing protein 1B (A) | C2D1B_RAT | 93818 | 34 | 3 |
| Splicing regulatory glutamine/lysine-rich protein 1 (C) | SREK1_RAT | 56930 | 34 | 3 |
| Zinc finger protein 57 (A) | ZFP57_RAT | 47584 | 34 | 2 |
| UPF0027 protein C22orf28 homolog (A) | CV028_RAT | 55727 | 33 | 1 |
| Scaffold attachment factor B1 (A, B,C) | SAFB1_RAT | 104960 | 33 | 4 |
| Transmembrane protein 109 (A,B) | TM109_RAT | 26282 | 33 | 2 |
| DNA topoisomerase 1 (A, C) | TOP1_RAT | 91159 | 32 | 3 |
| Hypoxia-inducible factor 3-alpha (A) | HIF3A_RAT | 73470 | 32 | 1 |
| Nucleoporin GLE1 (A) | GLE1_RAT | 79955 | 31 | 4 |
| Double-stranded RNA-specific editase 1 (A) | RED1_RAT | 78218 | 31 | 2 |
| Doublesex- and mab-3-related transcription factor C1 (A) | DMRTC_RAT | 24055 | 31 | 2 |
| Origin recognition complex subunit 1 (C) | ORC1_RAT | 96840 | 30 | 2 |
| 5′-AMP-activated protein kinase subunit gamma-1 (A) | AAKG1_RAT | 37534 | 30 | 1 |
| Transforming growth factor beta regulator 1 (A, C) | TBRG1_RAT | 45384 | 30 | 2 |
| High mobility group nucleosome-binding domain-containing protein 3 (C) |
HMGN3_RAT | 10176 | 30 | 1 |
| Histone acetyltransferase MYST2 (C) | MYST2_RAT | 71154 | 30 | 2 |
| Zinc finger and BTB domain-containing protein 44 (A, B) | ZBT44_RAT | 50987 | 30 | 3 |
| DNA (cytosine-5)-methyltransferase 1 (A, B) | DNMT1_RAT | 185054 | 30 | 5 |
| Nasal embryonic luteinizing hormone-releasing hormone factor (A) |
NELF_RAT | 60701 | 30 | 4 |
| Zinc finger and BTB domain-containing protein 24 (A) | ZBT24_RAT | 79355 | 29 | 3 |
| Nuclear pore complex protein Nup155 (A, B) | NU155_RAT | 156387 | 29 | 2 |
| Period circadian protein homolog 2 (B) | PER2_RAT | 137539 | 28 | 2 |
| Far upstream element-binding protein 2 (A, B) | FUBP2_RAT | 74466 | 28 | 1 |
| Paired box protein Pax-8 (A) | PAX8_RAT | 49118 | 28 | 1 |
| Coiled-coil domain-containing protein 55 (C) | CCD55_RAT | 64370 | 28 | 2 |
| PIN2/TERF1-interacting telomerase inhibitor 1 (A) | PINX1_RAT | 36916 | 28 | 2 |
| Coiled-coil domain-containing protein KIAA1826 homolog (A) | K1826_RAT | 41264 | 28 | 1 |
| Probable ATP-dependent RNA helicase DDX46 (A, C) | DDX46_RAT | 117826 | 28 | 6 |
| Lethal(3)malignant brain tumor-like protein 2 (A) | LMBL2_RAT | 80057 | 28 | 1 |
| DNA-directed RNA polymerase III subunit RPC3 (A) | RPC3_RAT | 60903 | 27 | 2 |
| DNA polymerase delta catalytic subunit (A) | DPOD1_RAT | 124948 | 27 | 5 |
| Nuclear pore complex protein Nup54 (A, C) | NUP54_RAT | 55825 | 27 | 1 |
| ATP-dependent RNA helicase DDX39 (A) | DDX39_RAT | 49591 | 27 | 4 |
| Histone deacetylase 4 (A, C) | HDAC4_RAT | 119377 | 27 | 3 |
| DNA topoisomerase 2-alpha (A) | TOP2A_RAT | 173853 | 27 | 6 |
| PDZ domain-containing protein 2 (A, C) | PDZD2_RAT | 296389 | 27 | 4 |
| Coiled-coil domain-containing protein 104 (A) | CC104_RAT | 39736 | 27 | 2 |
| Neurofibromin (C) | NF1_RAT | 320473 | 27 | 1 |
| Forkhead box protein J1 (C) | FOXJ1_RAT | 45994 | 27 | 2 |
| Origin recognition complex subunit 2 (A) | ORC2_RAT | 66094 | 27 | 3 |
| Transcriptional adapter 2-alpha (A, B) | TAD2A_RAT | 52107 | 27 | 2 |
| Telomerase protein component 1 (A, B,C) | TEP1_RAT | 295173 | 26 | 5 |
| Transcription factor EC (A, C) | TFEC_RAT | 35495 | 26 | 2 |
| SAP domain-containing ribonucleoprotein (A) | SARNP_RAT | 23647 | 26 | 2 |
| Transcription elongation factor A protein-like 8 (A) | TCAL8_RAT | 13553 | 26 | 1 |
| Protein AATF (A) | AATF_RAT | 59461 | 26 | 2 |
| DNA-directed RNA polymerase I subunit RPA2 (A) | RPA2_RAT | 129462 | 26 | 2 |
| Zinc finger CCCH domain-containing protein 18 (C) | ZCH18_RAT | 105702 | 26 | 2 |
| Transcription factor AP-2-alpha (A, C) | AP2A_RAT | 48316 | 26 | 3 |
| Neuronal PAS domain-containing protein 4 (C) | NPAS4_RAT | 88004 | 26 | 1 |
| Clusterin-associated protein 1 (B) | CLUA1_RAT | 46543 | 26 | 2 |
| Apolipoprotein A-IV (A) | APOA4_RAT | 44429 | 25 | 1 |
| 60S ribosomal protein L3 (A), B) | RL3_RAT | 46392 | 25 | 4 |
| 40S ribosomal protein S13 (A) | RS13_RAT | 17212 | 25 | 2 |
| Phosphatase and actin regulator 3 (A, B) | PHAR3_RAT | 58596 | 25 | 2 |
| Histone H3.3 (A) | H33_RAT | 15376 | 25 | 2 |
| SKI family transcriptional corepressor 1 (A) | SKOR1_RAT | 101312 | 25 | 3 |
| Protein GRINL1A (A, B) | GRL1A_RAT | 41305 | 25 | 1 |
| Cell growth-regulating nucleolar protein (A, C) | LYAR_RAT | 44110 | 25 | 2 |
| Pancreas/duodenum homeobox protein 1 (C) | PDX1_RAT | 30983 | 25 | 1 |
| DNA repair protein RAD50 (A, C) | RAD50_RAT | 154772 | 25 | 15 |
| RAC-gamma serine/threonine-protein kinase (B) | AKT3_RAT | 56217 | 25 | 2 |
| Leucine-rich repeat flightless-interacting protein 1 (C) | LRRF1_RAT | 80484 | 25 | 2 |
| Something about silencing protein 10 (C) | SAS10_RAT | 53949 | 24 | 1 |
| Structural maintenance of chromosomes protein 3 (A, B,C) | SMC3_RAT | 138761 | 24 | 4 |
| MACRO domain-containing protein 1 (Fragment) (C) | MACD1_RAT | 29081 | 24 | 2 |
| ZW10 interactor (C) | ZWINT_RAT | 30295 | 24 | 3 |
| Protein Dom3Z (A, C) | DOM3Z_RAT | 45784 | 24 | 2 |
| Kazrin (C) | KAZRN_RAT | 87433 | 24 | 3 |
| General transcription factor 3C polypeptide 1 (A, B,C) | TF3C1_RAT | 245004 | 24 | 3 |
| Suppression of tumorigenicity 18 protein (A) | ST18_RAT | 115074 | 24 | 2 |
| Myocyte-specific enhancer factor 2A (A) | MEF2A_RAT | 53391 | 24 | 2 |
| Homeobox protein MOX-2 (A) | MEOX2_RAT | 33869 | 24 | 1 |
| Ubiquitin carboxyl-terminal hydrolase 16 (A) | UBP16_RAT | 95242 | 24 | 3 |
| Sentrin-specific protease 2 (A) | SENP2_RAT | 67837 | 24 | 1 |
| Far upstream element-binding protein 1 (A, B) | FUBP1_RAT | 67326 | 24 | 3 |
| Hepatoma-derived growth factor-related protein 2 (A) | HDGR2_RAT | 74088 | 24 | 1 |
| 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-1 (A) |
PLCB1_RAT | 139113 | 24 | 1 |
| Selenocysteine insertion sequence-binding protein 2 (A,B,C) | SEBP2_RAT | 94154 | 24 | 5 |
| Protein salvador homolog 1 (B) | SAV1_RAT | 45133 | 24 | 3 |
| Structural maintenance of chromosomes protein 1A (A) | SMC1A_RAT | 143743 | 23 | 5 |
| DNA-binding protein SMUBP-2 (A) | SMBP2_RAT | 109400 | 23 | 3 |
| Myocyte-specific enhancer factor 2D (A, B) | MEF2D_RAT | 54563 | 23 | 3 |
| Nuclear pore membrane glycoprotein 210 (A, B) | PO210_RAT | 204943 | 23 | 2 |
| Myb-binding protein 1A (A) | MBB1A_RAT | 153046 | 23 | 4 |
| Fidgetin-like protein 1 (A) | FIGL1_RAT | 74891 | 23 | 1 |
| Smad nuclear interacting protein 1 (A) | SNIP1_RAT | 44953 | 23 | 1 |
| Aryl hydrocarbon receptor nuclear translocator 2 (A) | ARNT2_RAT | 78536 | 23 | 1 |
| H/ACA ribonucleoprotein complex subunit 1 (A, B) | GAR1_RAT | 23167 | 23 | 1 |
| Putative rRNA methyltransferase 3 (A, B,C) | RRMJ3_RAT | 95165 | 23 | 2 |
| REST corepressor 2 (C) | RCOR2_RAT | 58116 | 23 | 1 |
| PR domain zinc finger protein 2 (C) | PRDM2_RAT | 189890 | 23 | 2 |
| MutS protein homolog 5 (B,C) | MSH5_RAT | 93362 | 23 | 2 |
| E3 SUMO-protein ligase PIAS3 (C) | PIAS3_RAT | 69289 | 23 | 1 |
| Transcription factor IIIB 50 kDa subunit (C) | BRF2_RAT | 47421 | 22 | 2 |
| 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (C) | F263_RAT | 64491 | 22 | 1 |
| Vitamin D3 receptor (B) | VDR_RAT | 48467 | 22 | 2 |
| Meiosis-specific nuclear structural protein 1 (A, B) | MNS1_RAT | 61316 | 22 | 5 |
| Cell division cycle-associated protein 7 (A) | CDCA7_RAT | 43909 | 22 | 2 |
| Kinesin-like protein KIF22 (A, C) | KIF22_RAT | 73239 | 22 | 5 |
| Protein timeless homolog (A) | TIM_RAT | 139562 | 22 | 4 |
| U11/U12 small nuclear ribonucleoprotein 35 kDa protein (A) | U1SBP_RAT | 29186 | 22 | 2 |
| Nuclear pore complex protein Nup85 (A, B) | NUP85_RAT | 29186 | 22 | 2 |
| Interferon-stimulated 20 kDa exonuclease-like 2 (A) | I20L2_RAT | 41684 | 22 | 3 |
| ATPase WRNIP1 (A) | WRIP1_RAT | 72687 | 22 | 2 |
| Nucleolar RNA helicase 2 (A) | DDX21_RAT | 86540 | 22 | 4 |
| Period circadian protein homolog 1 (A, B) | PER1_RAT | 137562 | 22 | 2 |
| U4/U6.U5 tri-snRNP-associated protein 1 (A, B,C) | SNUT1_RAT | 91127 | 22 | 2 |
| Intraflagellar transport protein 172 homolog (C) | IF172_RAT | 199245 | 22 | 2 |
| Serine/threonine-protein kinase PRP4 homolog (A) | PRP4B_RAT | 117335 | 22 | 3 |
| CLK4-associating serine/arginine rich protein (C) | CLASR_RAT | 76932 | 22 | 3 |
| Nuclear pore complex protein Nup98-Nup96 (A) | NUP98_RAT | 198358 | 21 | 1 |
| Nucleolar GTP-binding protein 1 (A) | NOG1_RAT | 74640 | 21 | 3 |
| Protein SCAF8 (A) | SCAF8_RAT | 139874 | 21 | 2 |
| Myogenin (A, B,C) | MYOG_RAT | 33224 | 21 | 2 |
| Ester hydrolase C11orf54 homolog (C) | CK054_RAT | 35427 | 21 | 2 |
| Uncharacterized protein C1orf103 homolog (C) | CA103_RAT | 83074 | 21 | 4 |
| Poly(ADP-ribose) glycohydrolase (A, B) | PARG_RAT | 110417 | 21 | 1 |
| Dual specificity protein kinase CLK3 (C) | CLK3_RAT | 59247 | 21 | 2 |
| Scm-like with four MBT domains protein 1 (A, B,C) | SMBT1_RAT | 98984 | 21 | 1 |
| Chromodomain-helicase-DNA-binding protein 8 (A, B,C) | CHD8_RAT | 292449 | 21 | 1 |
| Digestive organ expansion factor homolog (C) | DEF_RAT | 88169 | 21 | 2 |
| Uncharacterized protein C12orf32 homolog (C) | CL032_RAT | 27281 | 21 | 1 |
| Regulator of G-protein signaling 14 (C) | RGS14_RAT | 59968 | 21 | 1 |
| Zinc finger CCHC-type and RNA-binding motif-containing protein 1 (C) |
ZCRB1_RAT | 24758 | 21 | 2 |
| Spliceosome RNA helicase Bat1 (B) | UAP56_RAT | 49460 | 21 | 1 |
| E3 SUMO-protein ligase PIAS2 (B) | PIAS2_RAT | 64303 | 21 | 1 |
A
GST-DEC specific interacting protein (see Methods) detected in pulldown samples using mitochondrial lysates from isolated cardiomyocytes.;
B
same as in A but using whole-cell cardiomyocyte lysates;
C
BKCa specific interacting protein (see Methods) detected in immunoprecipitation samples using monoclonal anti-BKCa antibody and lysates of Percoll purified mitochondria from left ventricle.