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. Author manuscript; available in PMC: 2017 Mar 10.
Published in final edited form as: Oncogene. 2016 Aug 29;36(10):1394–1403. doi: 10.1038/onc.2016.305

Figure 5.

Figure 5

Silencing Vimentin decreased cell invasion but not proliferation or migration. (A.) Cell counting kit 8 (CCK-8) was used for determination of cell proliferation between MVT-1 control (Ctrl) and MVT-1 Vimentin knockdown (VimKD) cells. 5,000 cells were plated in Complete Medium an analyzed for cell proliferation at 24, 48 and 72 hours, repeated twice. (B.) 150,000 MVT-1 Ctrl and MVT-1 VimKD cells were plated in 0.8μm inserts. The number of cells that migrated through the insert after 24 hours in Complete Medium was quantified by crystal violet staining, repeated three times. (C.) 400,000 MVT-1 Ctrl and MVT-1 VimKD cells were plated into 0.8μm inserts coated with matrigel. Cells were stimulated with PBS or 10nM Insulin for 24hours in Serum Free Medium. The number of cells that invaded through the matrigel-coated insert after 24 hours was quantified by crystal violet staining, repeated 3 times.