Skip to main content
. Author manuscript; available in PMC: 2017 Mar 10.
Published in final edited form as: Oncogene. 2016 Aug 29;36(10):1394–1403. doi: 10.1038/onc.2016.305

Figure 6.

Figure 6

Knockdown of Vimentin leads to decreased number of pulmonary metastases in the hyperinsulinemic mice. Wild type (10 per group) and MKR (13 per group) mice were injected with 100,000 of MVT-1 Control (Ctrl) and 100,000 of MVT-1 Vimentin KD (VimKD) into the 4th mammary fat pad. (A.) Tumor volumes were measured with calipers. Significant difference between the tumor sizes of MKR Ctrl and WT Ctrl group was observed. * p<0.05. (B.) Numbers of surface pulmonary macrometastases in WT and MKR mice. * p<0.05, as indicated. (C.) Tumor tissue was extracted and analyzed for Western Blot for Vimentin antibody. Representative blot is shown. Densitometry of Vimentin of representative blot n=3 WT Ctrl, n=4 WT VimKD, n=3 MKR Ctrl, and n=4 MKR VimKD. β-Actin antibody used as a loading control. Graphs represent mean per group and error bars are SEM.