Figure 6.
Numerous UV-Regulated Genes Are Antagonistically Regulated by the Long and Short ASCC3 Isoforms
(A) Hierarchical clustering of genes downregulated in UV-treated ASCC3 short knockout cells, shown alongside expression of the same genes in UV-treated ASCC3 Long knockdown cells, relative to expression in control cells. Blue and red bars indicate minimum and maximum log fold-changes, respectively.
(B) qRT-PCR analysis of IL7R and VEGFC expression in UV-treated short KO cells (light gray; top panel) compared to parental cells and in UV-treated long knockdown cells (dark gray; bottom panel) compared to NT shRNA cells (black) 20 hr after UV, shown as averaged GAPDH-normalized data, relative to untreated controls.
(C) As in (B) but analysis of genes with UV-induced expression.
(D) Rescue of gene expression in UV-treated short KO cells by transfection with siRNA targeting the long isoform. Analysis by qRT-PCR, with GAPDH-normalized data shown relative to UV-treated control cells.
(E) As in (D) but for global nascent transcription, as indicated by the low/high transcription ratio 20 hr after UV irradiation, relative to control cells. C, siNT control; P, parental control.