Figure 2.

Spheroid packing density decreases as spheroid size increases. (a) The predicted spheroid radius assuming maximal packing density was calculated and reported with the measured spheroid radii (n = 5). (b) As spheroid size increases, the packing density significantly decreases (n = 5). (c) DNA content increases linearly with the number of cells per spheroid (n = 3 per biological donor). (d) Normalized protein content decreases with increasing spheroid size (n = 3 per biological donor). (e) H&E staining indicates that the 15 000-cell spheroids (i) are more compact than the larger 30 000-cell (ii) and 60 000-cell spheroids (iii). Scale bar represents 250 µm. Groups with no significance are linked by the same letters, while groups with significance do not share the same letters. (Online version in colour.)