Table 1.
Methods for isolation and expansion of human corneal progenitors and their differentiation to corneal endothelial cells.
| Purpose | Cell Name | Isolation | Culture Cell Density | Growth Medium | Substrate | Time | References | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Basal Medium | Serum (%) | Growth Factors | Supplements | |||||||
| Expansion | HCEPs | Cell detachment Medium (Accutase) | 100-300 cells/cm2 | 1:1 DMEM/F12 | 20% KO Serum | 4 ng/ml bFGF | 20 µg/ml Laminin 511 | To sub-confluence | Hara, 2013 | |
| Differentiation | HCECs | Accutase | Passaged 1:2 | Low Glucose DMEM | 10% | FNC Mix | 3-4 Weeks | Hara, 2013 | ||
| Expansion | COPs | 400 U/ml type I collagenase | 1×105 Cells/cm2 |
1:1 DMEM/F12 | 20 ng/ml EGF, 20 ng/ml bFGF | B27 and N2 | None | Not found in the Paper | Hatou, 2013 | |
| Differentiation | HCECs | ? | 2×105 Cells/cm2 |
MEM | 1% | 5 ng/ml TGFβ2 | 1 mM all-trans retinoic acid, 1 mM GSK 3β inhibitor (6-bromoindirubin-3'-oxime), 10 mM ROCK inhibitor Y-27632, 1 mM insulin. | 0.1% gelatin; 1 µg/ml laminin; 1 µg/ml type I collagen | 1 week | Hatou, 2013 |