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. 2017 Jan 17;13(2):189–197. doi: 10.7150/ijbs.17386

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Inhibitory effect of ssc-miR-7134-3p on MARK4 fragments using the dual-luciferase system. (A-B) The assay was conducted with fragments spanning coordinates 2235 to 3007 and 871 to 1467 relative to the 5' end of the MARK4 transcription initiation site. (C) The assay was conducted with a fragment spanning coordinates 2434 to 2533 in which mutations had been introduced as follows: 2482wt-2502wt, 2482wt-2502mut, 2482mut-2502wt, and 2482mut-2502mut. (D) A 10 bp duplication was inserted between the two binding sites (2373/2533-R1), or downstream of binding site 2502 (2373/2533-R2). (E) The schematic diagram of ssc-miR-7134-3p binding at the two sites in MARK4. All relative luciferase activity values were normalized to the negative control mimics. **, p<0.01; *, p<0.05.