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Expression of MARK4 protein and mRNA in back fat tissue and primary fibroblast cells. MARK4 expression in back fat tissue by western blot (A) and qPCR (C). (B) Relative expression in intact and castrated pigs, based on the data in (A). (D) Expression of MARK4 detected by western blot in primary fibroblast cells transfected with ssc-miR-7134-3p and negative control. N.S., not significant. **, p<0.01.
