Figure 2.
Twist1 is up-regultated by hypoxia and promotes hypoxia induced radiorisistance. (A) Protein level of Twist1 in SiHa cells cultured under hypoxia for 0, 12, 24, 48, 72 and 96 h. (B) SiHa cells were treated by 100mM CoCl2 for 8h and western blot was used to detect Twist1 and HIF-1α expression. GAPDH was used as loading control. (C) The survival curve of control cells (Vector), Twist1-overexpression (Twist1+) and Twist1 knockdown (Twist1-) SiHa cells under normoxia or hypoxia conditions after irradiation was fitted by linear quadratic (L-Q) model. (D) Twist1+ and Twist1- SiHa cells were cultured under hypoxia and the effects of Twist1 expression level on HIF-1α was detected by western blot.