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. 2017 Jan 30;9(2):25. doi: 10.3390/v9020025

Figure 7.

Figure 7

Expression of genes related to virus recognition, antiviral response and amylase in mock-infected and virus-infected pancreatic acinar cells (A); Expression of genes related to virus recognition, antiviral response and islet hormone in mock-infected and virus-infected islets (B). Isolated human exocrine cell clusters and islets were mock-treated or infected with clinical strains of E6/91, E6/96, E6/12, E16 and E30 for 72 h. mRNA expression of interferon induced with helicase C domain 1 (IF1H1); 2′–5′-oligoadenylate synthetase 1 (OAS1); interferon-β (IFN-β); chemokine (C–X–C motif) ligand 10 (CXCL10); chemokine (C–C motif) ligand 5 (CCL5); amylase and insulin were assayed by real time reverse transcription-polymerase chain reaction (RT-PCR) and normalized by the house keeping gene 18S and β-actin using ΔCt method. Gene expression levels are presented as mRNA expression relative to the expression of the housekeeping gene (2−ΔCt). The expression level of each gene in virus-infected cells was compared to the one measured in mock-infected cells (control) from the same donors. Data are presented as means ± SD and were based on observations from at least three donors. Ct: cycle threshold. * p < 0.05, ** p < 0.01, *** p < 0.001.