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. 2017 Feb 21;9(2):36. doi: 10.3390/v9020036

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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A3R phage does not induce neutrophil degranulation. Neutrophil degranulation was evaluated based on changes of the expression of CD63 (primary granules) and CD66b (secondary granules) on neutrophils. A3R at the titer range 10⁶–10⁸ pfu/mL, phosphate-buffered saline (PBS) (the negative control), or suspension of heat-inactivated S. aureus cells (10⁸ cells/mL; the positive control) were added to whole blood samples. After 20-min incubation the percentage of CD63⁺ neutrophils (A), the mean fluorescence intensity (MFI) values of CD63 in the gated neutrophil population (B), the percentage of CD66b⁺ neutrophils (C), and the MFI values of CD66b in the gated neutrophil population (D) were determined by flow cytometry. The results shown are the mean percentage values of CD63⁺ and CD66⁺ neutrophils, as well as the MFI values of CD63 and CD66b in the gated neutrophil population ± SE in individual groups. Statistically significant differences (p < 0.05) between individual groups are indicated. The experiment was performed on blood samples from 13 blood donors.

A3R phage does not induce neutrophil degranulation. Neutrophil degranulation was evaluated based on changes of the expression of CD63 (primary granules) and CD66b (secondary granules) on neutrophils. A3R at the titer range 10⁶–10⁸ pfu/mL, phosphate-buffered saline (PBS) (the negative control), or suspension of heat-inactivated S. aureus cells (10⁸ cells/mL; the positive control) were added to whole blood samples. After 20-min incubation the percentage of CD63⁺ neutrophils (A), the mean fluorescence intensity (MFI) values of CD63 in the gated neutrophil population (B), the percentage of CD66b⁺ neutrophils (C), and the MFI values of CD66b in the gated neutrophil population (D) were determined by flow cytometry. The results shown are the mean percentage values of CD63⁺ and CD66⁺ neutrophils, as well as the MFI values of CD63 and CD66b in the gated neutrophil population ± SE in individual groups. Statistically significant differences (p < 0.05) between individual groups are indicated. The experiment was performed on blood samples from 13 blood donors.