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. 2017 Feb 10;7(2):37. doi: 10.3390/nano7020037

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Fluorescence spectra obtained after the incubation of BSA with PC or DMPC LUVs or MLs. (a) LUVs PC/Chol (8:2); (b) MLs PC/Chol (8:2); (c) LUVs DMPC/Chol (8:2); (d) MLs DMPC/Chol (8:2); (e) LUVs PC/Chol/PEG (8:2:0.3); (f) MLVs PC/Chol/PEG (8:2:0.3); (g) LUVs DMPC/Chol/PEG (8:2:0.3); (h) MLVs DMPC/Chol/PEG (8:2:0.3). The black line corresponds to the spectrum of 5 µM free BSA while the rest refer to the recordings after consecutive additions of 5 µL of 20 mM LUVs or MLs. The arrow indicates the direction of increase in LUVs or MLs concentration ranging from 62 to 370 µM into the reaction cuvette. λ_exc: 280 nm. The intensity of the emission band at 338 nm corresponding to the tryptophan was used for the calculation of the Stern-Volmer constant (K_SV) [51].

Fluorescence spectra obtained after the incubation of BSA with PC or DMPC LUVs or MLs. (a) LUVs PC/Chol (8:2); (b) MLs PC/Chol (8:2); (c) LUVs DMPC/Chol (8:2); (d) MLs DMPC/Chol (8:2); (e) LUVs PC/Chol/PEG (8:2:0.3); (f) MLVs PC/Chol/PEG (8:2:0.3); (g) LUVs DMPC/Chol/PEG (8:2:0.3); (h) MLVs DMPC/Chol/PEG (8:2:0.3). The black line corresponds to the spectrum of 5 µM free BSA while the rest refer to the recordings after consecutive additions of 5 µL of 20 mM LUVs or MLs. The arrow indicates the direction of increase in LUVs or MLs concentration ranging from 62 to 370 µM into the reaction cuvette. λ_exc: 280 nm. The intensity of the emission band at 338 nm corresponding to the tryptophan was used for the calculation of the Stern-Volmer constant (K_SV) [51].