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. 2017 Jan 26;8(2):50. doi: 10.3390/genes8020050

Figure 5.

Figure 5

Overproduction of RyfA1 has no significant impact on the growth of S. dysenteriae. An isopropyl β-D-1-thiogalactopyranoside (IPTG) inducible ryfA1 expression plasmid was created (pRyfA1) and introduced into S. dysenteriae. (a) Quantitative real-time PCR (qRT-PCR) analysis of the relative levels of RyfA1 present in S. dysenteriae carrying pRyfA1 or the pQE2 vector control following the growth of each strain in the presence of IPTG. RyfA1 levels were calculated using the ΔΔCt method [25] in which they are normalized to the level of rrsA measured in each sample and expressed relative to the level of RyfA measured in a single vector control sample. All data are the average of biological triplicate analyses and error bars represent one standard deviation. *** denotes a statistically significant difference with p < 0.0001. (b) Growth analysis of S. dysenteriae carrying the pQE2 vector control was compared to that of the strain carrying pRyfA1 under both non-inducing (0 μM IPTG) and inducing (20 μM IPTG) conditions. No significant differences were observed between the growth of S. dysenteriae carrying pRyfA1 or the vector control under either condition tested.