Figure 10.

Overproduction of RyfB1 does not impact plaque formation of S. dysenteriae. (a) Overproduction of RyfB1 by S. dysenteriae did not alter the number of plaques as compared to a vector control. (b) A monolayer of Henle cells was infected with S. dysenteriae containing a vector control or overproducing RyfB1 (pRyfB1). Overproduction of RyfB1 did not alter the plaque phenotype as from the vector control. (c) qRT-PCR was used to measure expression of RyfB1 and ompC. Significant overproduction of RyfB1 did not impact the relative levels of ompC transcript. * denotes a significant difference where p < 0.001. The relative levels of RyfB1 and ompC were calculated using the ΔΔCt method [25]. The housekeeping gene, rrsA, was used as an internal control.