Figure 4.

Transactive response DNA-binding protein (TDP-43) mislocalization and adenosine deaminase acting on RNA 2 (ADAR2) in anterior horn cells (AHCs) of spinal cord. Immunofluorescence assays revealed that ADAR2 (red) and TDP-43 (green) are both expressed in the nucleus of AHCs in wild type mice. TDP-43 immunoreactivity was absent in the AHCs of ADAR2 knockout mice (AR2; arrowhead). AHCs with low or no immunoreactivity to ADAR2 showed TDP-43 immunoreactivity in the cytoplasm and TDP-43-positive cytoplasmic aggregates in AR2 mice (arrow). TO-PRO-3 is a cellular maker (blue).