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. 2017 Feb 8;8(2):60. doi: 10.3390/genes8020060

Figure 5.

Figure 5

Choline acetyltransferase (ChAT) immunohistochemistry in the spinal cord of conditional adenosine deaminase acting on RNA 2 (ADAR2) knock-out mice (AR2) and AR2-rescued (AR2res) mice. (A) ChAT immunostaining (red) of the lumbar spinal cord. White dotted lines indicate the margin of the ventral gray matter. The bottom panels are the enlarged view of the upper panels. TO-PRO-3 (blue) was used as a cell marker. The scale bar indicates 20 µm; (B) As previously reported [19], the number of ChAT-positive anterior horn cells (AHCs) age-dependently decreases in AR2 mice compared to healthy controls. The age-dependent reduction in the number of ChAT-positive AHCs was rescued in AR2res mice. Means (columns) and standard errors of the means (SEMs; bars) are indicated. n = 5; * p < 0.05, ** p < 0.01, *** p < 0.001, Mann-Whitney U test against the value for wild type (WT) or AR2res groups.