Figure 1. SalB inhibit mitochondrial deformation induced by H₂O₂.

(A) Mitochondria from HL-7702 cells in the presence or absence of SalB were labeled with the mitochondrial-specific dye MTG for 30 min at 37 °C and viewed by fluorescence microscopy. Cells grown in normal medium exhibit long, tubular mitochondria; cells treated with H₂O₂ exhibit short, fragment mitochondria. SalB prevent H₂O₂-induced fragmentation and exhibit long, tubular mitochondria. (B) The graph depicts FF versus AR values for individual mitochondrion. Mitochondria of cells grown in normal medium have increased FF and AR values corresponding to long, tubular mitochondria. Fragmented mitochondria have reduced FF and AR values, n = 50 mitochondria. (C) The mitochondrial number of cells grown in H₂O₂ is significantly decreased compared with normal cells. (D,E) As mitochondrial morphological parameters, AR and FF were quantified by Image software. Images from a total of 15 individual cells were analyzed through three independent experiments. *P < 0.05 versus H₂O₂-treated control.