Figure 6. SalB banlances mitochondrial fusion-fission through up-regulate mortalin in primary hepatocyte.

(A) Western blot analysis showed the expression of mortalin was higher in SalB treated groups compare to control group. β-actin was used to normalize protein loading. The representative of three experiments was shown. Full-length blots are presented in Supplementary Figure 5. (B) Quantitative results from Western blots (n = 3, *P < 0.05). (C) Confocal fluorescence microscope (60×) images showed that treatment with H₂O₂ induced mitochondrial fission, while pretreatment with 50, 100, 200 μM SalB can inhibit mitochondrial structure instability induced by H₂O₂. (D) The values of FF versus AR for individual mitochondrion, n = 50. (E,F) AR and FF were mitochondrial morphological parameters, treatment with SalB augmented AR and FF values, which means increased number of long tubular mitochondria. Images from a total of 15 individual cells were analyzed through three independent experiments, *P < 0.05.