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. 2017 Mar 2;7:43736. doi: 10.1038/srep43736

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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HC-HA/PTX3 purified from three donor lots (10 μg of HA each) was digested without (−) or with (+) hyaluronidase (1 U/μg HA) at 37 °C for 1 h and analyzed by Western blot for HC1 (A, using purified human IαI as the control that released HC1 after being treated with 100 mM NaOH at ~25 °C for 1 h), and PTX3 (B, using human recombinant PTX3 as the control). For examining the stability, lyophilized HC-HA/PTX3 was stored at −80 °C for 6 months (6 m) and 12 months (12 m), followed by Western blot (as described above) to check the integrity of HC1 (A) and PTX3 (B). Note: for the clarity, some unused blank lanes were cut off. The full images of all blots were present and exposure (brightness and contrast) was applied to the whole image.