Figure 5. Inhibition of RPE cell migration and collagen gel contraction by HC-HA/PTX3.
(A) (migration). ARPE-19 cells (2 × 106/ml) treated with PBS (vehicle control), HA (25 μg/ml), or HC-HA/PTX3 (25 μg/ml) were in the upper compartment while DMEM/F12 without or with EGF (10 ng/ml), FGF-2 (20 ng/ml), and TGF-β1 (10 ng/ml) were in the lower compartment of transwell wells. After incubation for 8 h, the number of migrated cells were counted from six random microscopic fields (n = 4, *indicates p < 0.05 when compared with PBS + EGF + FGF-2 + TGF-β1). (B) (collagen gel contraction). ARPE-19 cells or primary human RPE cells (5 × 105/ml) without or with TGF-β1 (10 ng/ml) and treated with PBS (vehicle control), HA (25 μg/ml), or HC-HA/PTX3 (25 μg/ml) were on the top of collagen gel. The percentage of gel contraction was determined by measuring the gel size at 72 h when compared to the initial size (at 0 h) in each group (n = 4, * indicates p < 0.05 compared with PBS + TGF-β1).