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ARPE-19 cells seeded at 1 × 104/cm2 were simultaneously treated with EGF (10 ng/ml) and FGF-2 (20 ng/ml) or TGF-β1 (10 ng/ml) together with PBS, HA (25 μg/ml), or HC-HA/PTX3 (25 μg/ml) for 4 h. The mRNA expression was measured by the real-time PCR assay. n = 4, *indicates p < 0.05 vs. PBS + EGF + FGF-2 or PBS + TGF-β1.
