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. 2017 Mar 2;7:43729. doi: 10.1038/srep43729

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Alcian blue staining (A,D) and immunostaining for type II collagen (B,E) and type X collagen (C,F). The scale bars show 100 μm. Quantitative real-time reverse transcription-polymerase chain reaction analysis of the chondrogenic differentiation process (G–L) (G) type II collagen mRNA, (H) aggrecan mRNA, (I) type X collagen mRNA, (J) Runx2 mRNA, (K) Runx3 mRNA, (L) Ihh mRNA. All values are expressed as the mean ± SEM. (*P < 0.05, **P < 0.01).