Skip to main content
. 2017 Mar 2;7:43711. doi: 10.1038/srep43711

Figure 2. PF-46396 is active against HIV-1 clade C.

Figure 2

CA-SP1 accumulation assay of (a) HIV-1 clade B clone NL4-3 and (b) HIV-1 clade C clones in the presence of PF-46396. HEK-293T cells were transfected with (a) HIV-1 clade B clone NL4-3 or (b) HIV-1 clade C clones K3016, IndieC1 and ZM247. Cells were treated with increasing concentrations of PF-46396 (0.1–5.0 μM) or with DMSO only. Cell- and virion-associated Gag proteins were detected by western blotting. Positions of Pr55Gag, p41, CA, and CA-SP1 are indicated. Gel images shown here are representative of three independent experiments. Quantification of virion-associated % CA-SP1 relative to total CA + CA-SP1 is presented in the graphs. Error bars indicate standard deviations. (c) Replication kinetics of HIV-1 clade C clone K3016 in the presence or absence of PF-46396. HUT-R5 T-cells were transfected with K3016 WT and propagated in the presence of 0–3.0 μM of PF-46396. Virus replication was monitored by quantifying HIV-1 p24 concentration in the culture supernatants. This is a representative of three independent experiments.