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. 2017 Mar 2;7:43711. doi: 10.1038/srep43711

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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CA-SP1 accumulation assay of (a) HIV-1 clade C K3016 WT, K3016 A1V, I201V and H226Y mutants (b) HIV-1 clade C K3016 WT, K3016 G156E, P157S and P160L mutants (c) HIV-1 clade C K3016 WT, K3016 G225D, A3V and A3T mutants. HEK-293T cells were transfected with HIV-1 clade C clone K3016 WT and indicated K3016 mutants. Cells were treated with PF-46396 (1.0 & 5.0 μM) or with DMSO only. Cell- and virion-associated Gag proteins were detected by western blotting. Positions of Pr55^Gag, p41, CA, and CA-SP1 are indicated. Lanes are numbered at the bottom of the gel. Gel images shown here are representative of three independent experiments. Quantification of virion-associated % CA-SP1 relative to total CA + CA-SP1 is presented in the graphs on the right side of each panel. (d) Relative virus release efficiency (VRE) of PF-46396-resistant mutants in the absence and presence of the compound. VRE is calculated as % of [virus-associated CA + CA-SP1]/[total (cell + virus-associated) Gag] relative to K3016 WT from (a–c) gel images. Error bars indicate standard deviations from three independent experiments.