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. 2017 Mar 2;7:43711. doi: 10.1038/srep43711

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The HUT-R5 T-cells were transfected with K3016 mutants (a) A1V, (b) I201V, (c) H226Y, (d) G156E, (e) P157S, (f) P160L, (g) G225D, (h) A3V and (i) A3T. K3016 WT was also included as an experimental control. Transfected cells were propagated in the presence of 2.0 & 3.0 μM of PF-46396 or with DMSO only. Virus replication was monitored by quantifying HIV-1 p24 concentration in the culture supernatant. This is a representative of two independent experiments.