Figure 6. APA influences viral infection.

(a) siRNAs were transfected into A549 cells, and the interference efficiency was determined with qRT-PCR at 48 h. (b) We transfected A549 cells with individual siRNAs for 48 h and infected them with VSV-eGFP at an MOI of 1 for 6 h or 12 h. Then, qRT-PCR was performed to detect VSV replication at the mRNA level. Data are mean±s.d. *P<0.05; **P<0.01. (c) FACS analysis of A549 cells transfected with control siRNA (siCon) or the indicated siRNAs and then infected with VSV-eGFP at an MOI of 2 for 6 h. FACS gating strategies from one representative experiment have been provided as Supplementary Fig. 10. (d) A549 cells were transfected with individual siRNAs for 48 h and infected with VSV at an MOI of 1 for 12 h. Then, western blotting was performed to detect VSV replication at the protein level. The uncropped scans of western blots have been provided as Supplementary Fig. 11. (e) Relative mRNA expression of the core 3′ processing factors in VSV-infected MDMs. Data were obtained from two biological duplicates of IVT-SAPAS. The x axis denotes six time points post infection and the y axis denotes relative expression. (f) Relative 3′ UTR length of the core 3′ processing genes with switched poly(A) sites during the antiviral immune response. Data were obtained from IVT-SAPAS. The x axis denotes six infection time points and the y axis denotes e UTR/c UTR, which represents the ratio of longer 3′ UTR isoforms to total mRNA isoforms.