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. 2017 Feb 24;8:14577. doi: 10.1038/ncomms14577

Figure 3. NEKO mice display nephrogenic diabetes insipidus.

Figure 3

(a) Plasma AVP level in control and NEKO mice. (b) Relative Avp gene expression level in the hypothalamus of control and NEKO mice. Data are the means±s.e. (n=4). (*P<0.05, unpaired t-test) (c) Urine volume of control and Keap1-NKO mice. (d) Western blotting analysis of AQP2 protein in the kidney of control and NEKO mice. Open and closed arrowheads indicate glycosylated and non-glycosylated forms of AQP2, respectively. The molecular weight standards are shown on the right. (e) Graphical representation of total AQP2 protein level shown in d. Data are the means±s.e. (n=3). (*P<0.05, unpaired t-test) (f) Relative Aqp2 gene expression level in the kidney of control and NEKO mice. Data are the means±s.e. (n=7). (g,h) AQP2 immunostaining of kidney sections of control and NEKO mice. Insets show a higher magnification view of the collecting duct. Scale bar, 10 μm.