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. 2017 Mar 2;7:43509. doi: 10.1038/srep43509

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Blood flow was measured by LSFG before and immediately after occlusion, and on days 1, 3, 7, 14, and 30 to verify the duration of the reduction of blood flow. Blood flow was significantly reduced compared to the pre-treatment group 1, 3, 7, 14, and 30 days after occlusion. The data are expressed as means ± S.E.M. (n = 5–8). ^##P < 0.01 vs pre-treatment group (Dunnett’s test).