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. 2017 Mar 2;7:43905. doi: 10.1038/srep43905

Figure 5. Schematic of Paper-ELISA representing the steps of the detection process.

Figure 5

In this sandwich ELISA, identical circular regions with hydrophobic boundaries were marked on ZnO-NRs/WFP; the primary antibodies were immobilized on ZnO paper for 10 min.; four different concentrations of antigen were allowed to bind for 10 min.; HRP-conjugated secondary antibodies were allowed to bind for 1 min. followed by a quick wash with PBS and then TMB was added to react with the enzyme HRP for 30 min and produce colour. The strips were scanned and the colour intensity and spectra were analyzed using imageJ (version 1.45).